| Dr. Masoud Tohidfar |
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Position
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Academic staff (assistant professor) of Agricultural Biotechnology Research Institute of Iran (ABRII), Department of Tissue Culture and Transformation. Permanent employee of AREO.
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Address
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Department of Tissue culture and Gene Transformation
Agricultural Biotechnology Research Institute of Iran (ABRII)
Seed and Plant Improvement Institutes Campus
P. O. Box: 31535-1897
Mahdasht Road, Karaj, Iran
Phone: +98 261 2703536
Fax: +98 261 2704539
Email: Tohidfar@abri.ac.ir
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Date of Birth
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04.04.1968 in Gorghan, Iran
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Academic Carrier
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2002:
PhD in Plant Biotechnology, Tehran University, Iran
Thesis: Genetic engineering of cotton for enhanced resistance against fungal disease, verticilliosis and fusariosis
1996:
M.Sc in Plant breeding, Tehran University, Iran
1992:
B.Sc Agronomy and plant breeding, Gilan University, Iran
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Science courses
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- Workshop "Wheat rust in the centeral and West Asian Countries S.P.I.I.", Karaj, Iran, 19-23 May, 1996
- Workshop "Plant Transformation" Tehran Oct 4-12, 1997
- Workshop "Transformation and Molecular Analysis of Transgenic Plant", Karaj -17-27 August 1998. By IRRI
- Workshop "Regional Workshop on Latest Application of Biotechnology for Agriculture Development. 22-28 August 1999
- Workshop "Recombinant DNA" Karaj 24-28 Sep by ABRII and NRCGEB
- Workshop "Bioinformatics" 8-12 December 2002, Karaj, Iran
- Workshop Aplication of molecular marker in crops,Karaj 29Feb-3March 2003 by ABRII
- Workshop Intelegdual property rights, Syria 23July-25July 2003 by ICARDA
- Teaching in a workshop on Rice Transformation, 23 to 27, 2004, Karaj, Iran
- Workshop for drafting NBF, Tehran, Iran, 10 to 12 July, 2004
- Workshop on Biosafety, Tehran, Iran, 1 Sep, 2004
- Lecture at the workshop on Analysis of Transgenic Plants, 26 Feb-2Mar 2005, Iran, Karaj
- Lecture at the workshop on Introduction to Biosafety, 26 January 2005, Iran, Karaj, ABRII
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Research Area
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- Plant Molecular Biology and Plant Biotechnology
- Study association between seed storage protein (Glutenin) and bread making quality in advanced breeding lines of wheat using SDS-PAGE
- Plant regeneration via somatic in four cultivars of cotton (Gossypium hirsutum L)
- Agrobacterium-mediated transformation of cotton using a gus gene
- Produced transgenic cotton contaning CryIA(b) gene drived from hypocotyl
- Produced transgenic cotton contaning Chitinase gene drived from hypocotyl
- Transformation of a cotton cultivar by using Agrobacterium and the shoot apex
- Study association high molecular weight glutenin subunits in wheat with some important quality triats by using multivariant analysis
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Research experience
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- Molecular Biology Techniques: Isolation & purification of DNA; construction of plasmid; gene cloning; Southern, western analyses; gene expression; polymerase chain reaction techniques
- Plant Tissue Culture: shoot apex, somatic embryogenesis, regeneration and plant transformation techniques
- Isolation of plasmide
- Mini-preparation of plasmide
- Maxi-preparation of plasmide
- Spectrophotometric determination of DNA
- Restriction digestion of cloned DNA
- Agarose gel electrophoresis of DNA
- Extraction of DNA fregments from agarose gels
- DNA purification by Ethanol precipitation of DNA
- Ligation of vector and target DNAs
- Preparation of competent cell
- Transformation of competent cells
- Transformation of Agrobacterium
- Colony screen of Pgem vector transformation
- Histochemical Analysis of GUS Activity
- Agarose gel DNA Extraction Kit
- Measureing chitinase enzyme activity
- Westhern Analysis
- Southren Analysis
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Language
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Farsi, English
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Awards
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- Research Scholar (Present): Scientific member of Agricultural Biotechnology Research Institute of Iran
- PhD Fellowship (1997)
- During the PhD program (average 18.8 out of 20) award for high average mean (thesis and course work) from the Ministry of Jihad-e-Agriculture
- M.Sc Fellowship (1995)
- 5% of all student during the M.Sc program (average 17 out of 20) frist student
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Publications
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Scientific Journals
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Tohidfar M,C. Abdimisani and B. Yazdi Samdi (1998)
Association betwwen seed storage protein (Glutenin) and bread making quality in advanced breeding lins of wheat vua SDS-PAGE
Iranian journal of Agriculture Sciences.Vol .29 No.3 735-742. (Abstract English)
Tohidfar M., C. Abdimisani B. Yazdi Samdi (1999)
Association high molecular weight glutenin subunits in wheat with some important quality triats by using multivariant analysis"
Iranian journal of Agriculture Sciences.Vol .30 No.3. 854-861. (Abstract English)
Tohidfar M., M. Mohammadi, and C. Abdimisani (2003)
Plant regeneration via embryogesis in four cultivars of cotton
Seed and plant, Vol. 17 (4) 376-385. (Abstract English)
Tohidfar M., B. Ghareyazie, M. Mohammadi, C. Abdmishani (2003)
Agrobacterium-mediated transformation of cotton using a chitinase gene
Journal of Science of the Universitry of Theran Vol . 30 (3) 367-380 (Abstract English)
Tohidfar M, M. Mohammadi, B. Ghareyazie and S. Abolghasem Mohammadi (2004)
Relationships Between High Molecular Weight Subunits of Glutenin and Breadmaking Quality of 280 Advanced Lines in Crossbred Wheat
Cereal Food World. Feb. 49:28-34
Tohidfar M., B. Ghareyazie, M. Mohammadi, C. Abdmishani (2005)
Agrobacterium-mediated transformation of cotton using a chitinase gene
Plant Cell Tissue and Organ Culture. 83: 83-96
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Conferences and Congresses
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Tohidfar M.,and C.Abdmishani
Association between seed storage protein (Glutenin) and bread making quality in advanced breeding lines of wheat via SDS-PAGE
3rd International crop science congress 2000, 17-22 August Hamburg, Germany
Tohidfar M.,and C.Abdmishani
Association of high molecular weight glutenin subunits of wheat with some important quality traits using multivariae analysis
3rd International crop science congress 2000 ,17-22 August Hamburg, Germany
Tohidfar M.
Using of multivariate analysis method in protein markers and breadmaking quality in wheat
In the 4 th Iranian International Statistical Conference Shahid Behiesti University August 23-25 1998
Tohidfar M., Mohammadi M. and B. Ghareyazie (2005)
Agrobacterium-mediated transformation of cotton (Gossypium hirsutum L.) using a heterologous bean chitinase gene
Biological in Asia International Conference 2004, 7-10 December, Singapoure
Tohidfar M., C. Abdimisani B. Yazdi Samdi
Association between seed storage protein (Glutenin) and bread making quality in advanced breeding lines of wheat via SDS-PAGE
3rd International crop science congress 2000 ,17-22 August Hamburg, Germany
Tohidfar M., C. Abdimisani B. Yazdi Samdi
Association of high molecular weight glutenin subunits of wheat with some important quality traits using multivariate analysis
3rd International crop science congress 2000 ,17-22 August Hamburg, Germany
Tohidfar M., C. Abdimisani B. Yazdi Samdi
Relationship between high molecular wight glutenin subunits with SDS sedimentation test to estimate the backing quality of early generation of bread wheat under selection
Second National Biotechnology Congress Islamic Repuplic of Iran-Oct. 9-11, 2001, Karaj
Tohidfar M.
Selectable marker and mechanisms for production marker-free transgenic plants
Second National Biotechnology Congress Islamic Repuplic of Iran-Oct. 9-11 ,2001, Karaj
Tohidfar M.. M. Mohammadi and C. Abdmishani
Effects of genotype and growth medium on callus formation and regeneration of cotton
In the first National Congress of Biotechnology Islamic Repuplic of Iran University of Tarbeyat Modarrss Feb 1998
Tohidfar M.,and C. Abdimisani
Using of multivariate analysis method in protein markers and breadmaking quality in wheat
In the 4 th Iranian International Statistical Conference Shahid Behiesti University August 23-25 1998
M.Kalathe and Tohidfar M.
Wheat cultivation during summer and its application in breeding and production and varities
In the Iranian congress of crop production and plant breeding Sep 4-9 1998 Karaj
Tohidfar M., B. Ghareyazie,and M. Mohammadi (2002)
Agrobacterium-mediated transformation of cotton using a gus gene
In the 7th Iranian Crop Science Congress Aug 24-26 Karaj, Iran
Zaferanlo,B, Tohidfar M. and B. Ghareyazie (2002)
Agrobacterium-mediated transformation of cotton using a P.I (Kunitz trypsin inhibitor) gene
In the 7th Iranian Crop Science Congress Aug 24-26 Karaj, Iran
Tohidfar M., B. Ghareyazie, M. Mohammadi, C. Abdmishani (2002)
Agrobacterium-mediated transformation of cotton using a chitinase gene
In 15th Iranian Plant Protection Congress 7-11 Sep. Kermanshah, Iran
Tohidfar M., B. Ghareyazie, M. Mohammadi, C. Abdmishani (2002)
Agrobacterium-mediated transformation of cotton using a CryIA(b)gene
In 15th Iranian Plant Protection Congress 7-11 Sep. Kermanshah, Iran
Tohidfar M. B. Ghareyazie, M. Mohammadi (2003)
Genetic engineering of cotton for enhanced resistance against fungal disease, verticilliosis and fusariosis
In the 3rd National Congress of Biotechnology Islamic Repuplic of Iran University of Mahshad 7-10 Sep 2003
Farhadi, L. C. Abdmishani. Tohidfar M., M. Mohammadi (2003)
Agrobacterium-mediated transformation of tobacco using a chitinase gene
In the 3rd National Congress of Biotechnology Islamic Repuplic of Iran University of Mahshad 7-10 Sep 2003
Tohidfar, M. and L. Ramzanpour
Transformation of Cotton Using a gus Gene and Study of its Inheritance and Expression in Second Generation (T1) of Transgenic Cotton
In The 8th Iranian Crop Production & Breeding Congress Aug 25-27. 2004. The University of Gillan, Rasht. Iran
Tohidfar, M. Kaviyani, M. (2005)
Transgenic, Pest management, and the Environment
In First National Biosafety Congress of Iran. Karaj. Feb. 16-17
Tohidfar, M. and Darzeyani, G. (2005)
Risk Assessment of Bt Cotton
In the First National Biosafety Congress of Iran. Karaj. Feb. 16-17
Tohidfar, M. (2005)
Guideline for the conducting safety assessment of food transgenic plants
In the First National Biosafety Congress of Iran. Karaj. Feb. 16-17
Tohidfar, M. and Darzeyani, G. (2005)
Risk Assessment of transgenic Cotton
In the First National Biosafety Congress of Iran. Karaj. Feb. 16-17
Tohidfar, M. Behnia, S. Ghareyazie, B. (2005)
Biotechnology and improvement of forage crop. In the First National forage crop Congress of Iran. University of Tehran, Karaj. Aug 9-11
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Books
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Tohidfar, M. (2003)
Application of mean comparition and ortogonal polynomial in exprimental desig
Press, Agricultural Research Extrention and Education. 75P
Tohidfar, M. and Ghareyazie B. (1384)
Risk assesment genetically modiefied plant
(in press)
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