| Study of Promoter Activity Pattern of OsHKT1 Gene in Different Tissues of Rice and Determination of Effect of Drought Stress and Sodium, Potassium, Rubidium and Ammonium Ions on the Promoter Activity |
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In this study a plasmid (pCproHKT1) containing the gus reporter gene under the control of promoter region of OsHKT1 gene was constructed. Transient expression of the gus gene in callus, leaf, and root of rice plants under one level of NaCl stress (150 mM) and control (0 mM) was studied using the biolistic method. Transient gene expression was only detectable in leaf tissue under both treatments but the expression was less in 150 mM NaCl than control. In order to study of gene expression pattern under Na, Rb, Li, and NH4 ions effects transformation of rice callus was performed by pCproHKT1. 13 plants were regenerated on selection media containing 50 mg l-1 hygromaycin B. PCR-based analysis carried out using 4 primer pairs showed that genome of all plants have at least a part of the plasmid. Plant No. 10 (T0/10) was contained the gus gene after the OsHKT1 promoter. According to our results promoter of the OsHKT1 gene was not suitable for practical use in genetic engineering of crops because this promoter could not induce high expression of introduced genes in rice especially under salt stress. More basic studies however by using transformed plants are possible for understanding of mechanisms involved in regulation of the OsHKT1 expression under ions stress.
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