| Development of PCR based marker for candidate Fusarium head blight resistance gene (PDR5) in wheat using a 'Wangshuibai'-derived population |
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Fusarium head blight (FHB, scab), predominantly caused by Fusarium graminearum and F. culmorum, has become a major limiting factor for sustainable wheat (Triticum aestivum L.) production around the world. Although diverse sources of resistance to FHB are available in wheat, breeding for FHB resistance is tedious. The major goal of this study was to develop gene-specific markers for a candidate FHB resistance gene, PDR5 (pleiotropic drug resistance 5), encoding ABC transporter protein and detoxifying cells using ATP hydrolysis for QTL mapping of FHB resistance in wheat. Mapping populations were developed from a cross of parent 'Wangshuibai' (resistant), by parent 'Seri82' (susceptible). Parental lines and SSD lines in F3, F5 and F7 generations were inoculated under field conditions. SSD lines in the F3 generation were genotyped using AFLP and SSR markers and the primers designed based on the sequence aligned between PDR5-type ABC transporter homologues gene. Genotypic and phenotypic data were used for single, linkage map and QTL analyses. Single marker analysis indicated that one designed primer pairs had a significant negative effect on FHB severity. The results of linkage group and QTL analyses will be discussed.
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