| Molecular breeding for improved Fusarium head blight resistance in wheat in Iran |
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Molecular breeding for improved FHB resistance in wheat in Iran was started in 2002 by participating in scientific co-operation projects between Iran and Austria. The main objective of this work was to identify molecular markers linked to FHB resistance for marker assisted backcross (MAB) breeding with the aim to develop locally adapted wheat cultivars with improved Fusarium resistance in much shorter time than by conventional breeding. Different types of populations segregating and DNA markers (SSR, AFLP and RGA) were used for QTL mapping of FHB resistance. The preliminary QTL analyses results showed one consistent QTL for FHB resistance was identified on 3BS chromosome in both 'Wangshuibai' and 'Sumai-3' populations segregating. Three consistent QTLs for FHB resistance on chromosomes 1BL, 3AL and 7AS were detected on 'Frontana' populations segregating. Strong emphasis was placed on mapping candidate genes (RPL3 and PDR5-like) and ESTs putatively involved in FHB resistance. The preliminary single marker analysis indicated that one gene-specific marker for PDR5-like genes had a significant negative effect on FHB severity in 'Wangshuibai' derived populations. Current work is transferring individual detected QTLs and markers associated with FHB resistance into local adapted cultivars. Furthermore, these markers will be used to test if there are any differences among the local FHB resistance Iranian sources with the widely used Chinese and Brazilian sources for the markers/genes linked to FHB resistance. Other works in progress are including identifying specific FHB infection responsive genes/proteins using transcriptome analysis (cDNA-AFLP) and two-dimensional gel electrophoresis (2D-GE) in our developed RILs and NILs.
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