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April 19, 2024
Fa
dividerFaculity MemberdividerSaeid Kadkhodaei
Agricultural Biotechnology Research Institute of Iran
Assistant Professor
Saeid Kadkhodaei
Assistant Professor
tel +98-3142235858
email s.kadkhodaei@abrii.ac.ir
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  • Introduction
  • Research
  • Science Articles

Introduction:

Dr. Saeid Kadkhodaei joined Agricultural Biotechnology Research Institute of Iran (ABRII)-genomics department in 2002. He received his PhD in genetic engineering from University Putra Malaysia in 2014 and two postdoctoral research grants from UPM and National Elites Foundation (Isfahan University of Technology) working on bioinformatics and CRISPR-based genome engineering, respectively. His current research interest lies on the development of CRISPR-based diagnostics.

 

Education:

  • Post doctorate

Isfahan University of Technology, Isfahan, Iran, 2017-2018

Thesis: “Genome engineering of yeast through CRISPR/Cas9 toward development of protease deficient strains”

  • Post doctorate

Universiti Putra Malaysia, 2015-2016

Thesis: “Transciptome profiling at early infection of Elaeis guineensis by Ganoderma boninense provides novel insights on fungal transition from biotrophic to necrotrophic phase”

  • PhD

Universiti Putra Malaysia, 2009-2014

Thesis: “Creation of a series of potential gene cassettes for recombinant protein expression in green microalgal system”

  • MS

Tabriz University, Tabriz, Iran, 1999-2002

Thesis: “Genetic identification of Iranian wild almonds using molecular markers”

  • BS

Isfahan University of Technology, Isfahan, Iran, 1994-1998

 

Honor, Awards, and Scholarships:

  • Shahid Chamran PostDoc Fellowship, 2017, National Elites Foundation
  • Gold Medal in the Invention, Research and Innovation Exhibition (PRPI 2014), Malaysia, 2014
  • Bronze Medal in the Invention, Research and Innovation Exhibition (PRPI 2014), Malaysia, 2014
  • Distinguished PhD student among the Iranian students of Malaysian universities in Basic Sciences, 2013
  • International Graduate Research Fellowship (IGRF), 2011, Universiti Putra Malaysia
  • Prize for the Best Book of the Year (Graduate Students), 2010, Ministry of Culture, Iranian Students Book Agency
  • Distinguished Researcher in ABRII-CR (Agricultural Biotechnology Research Institute of Iran), 2009
  • Distinguished Researcher in ABRII-CR (Agricultural Biotechnology Research Institute of Iran), 2008
  • Certificate of appreciation for Developing Biotechnology Related Softwares, 2007, Iranian Biotechnology Society

 

Research Interest:

  • CRISPR-based diagnostics
  • Synthetic biology
  • CRISPR-based molecular detection of potato X virus via qPCR, RT-LAMP and CRISPR diagnostics 

Publications

Books/Book chapters:

  • Genome Editing Using CRISPR Technology and Its Applications in Biosciences (in Persian)-Tarbiat Modarres University Publication, 2021
  • Cis/Transgene Optimization: Systematic discovery of novel gene expression elements using bioinformatics and computational biology approaches-Springer, 2018
  • An Introduction to Synthetic biology: Design and Assembly of Genetic Constructs (eBook-in English), 2015
  • Molecular Farming (in Persian), 2010
  • An Introduction to Genetic Engineering Techniques (eBook-in Persian), 2009
  • An Introduction to Molecular Markers (eBook-in Persian), 2009
  • GLP (Good Laboratory Practice) (as a joint editor-in Persian), 2006
  • Sorghum (as scientific and literary editor-in Persian), 2009
  • Glossary of Plant Tissue Culture (as scientific and literary editor-in Persian), 2004

 

Research papers:

  1. Talei D, Khayam Nekouei M, Kadkhdodaei S. 2022. Evaluation of genetic diversity of Nain Havandi plant masses based on protein and SRAP markers. JMBS. In Press.
  2. Hosseini MS, Ebrahimi M, Abadia J, Kadkhodaei S, et al. 2022. Growth, phytochemical parameters and glycyrrhizin production in licorice (Glycyrrhiza glabra L.) grown in the field with saline water irrigation. Industrial Crops and Products 177, 114444. https://doi.org/10.1016/j.indcrop.2021.114444
  3. Bande F, Arshad SS, Bejo MH, Omar AR, Moeini H, Kadkhodaei S, et al.. Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus. 2020. Microbial Pathogenesis. 149. 104560. 10.1016/j.micpath.2020.104560
  4. Talei D, Khayam Nekouei M, Mardi M, Kadkhdodaei S. 2020. Improving productivity of steviol glycosides in Stevia rebaudiana via induced polyploidy. Journal of Crop Science and Biotechnology. 23(4). https://doi.org/10.1007/s12892-020-00038-5
  5. Khayam Nekouei M, Moazam M, Mardi M, Kadkhdodaei S. 2020. Development of SSR Markers Associated with Biosynthesis Pathway of Steviol Glycosides in Stevia through De Novo Transcriptome Assembly. . JMBS. 2020; 11 (2) :185-191
  6. Monfared HH, Chew JK, Azizi P, Xue GP, Ee S, Kadkhodaei S, Hedayati P, Ismail I, Zainal Z. 2020. Overexpression of a Rice Monosaccharide Transporter Gene (OsMST6) Confers Enhanced Tolerance to Drought and Salinity Stress in Arabidopsis thaliana. Plant Mol Biol Rep. doi:10.1007/s11105-019-01186-x
  7. Bahari MNA, Sakeh NM, Abdullah SNA, Ramli RR, Kadkhodaei S. 2018. Transciptome profiling at early infection of Elaeis guineensis by Ganoderma boninense provides novel insights on fungal transition from biotrophic to necrotrophic phase. BMC plant biology 18 (1), 377. doi:10.1186/s12870-018-1594-9
  8. Kadkhodaei S, Hashemi F, Rezaei M, et al. 2018. Conceptual Models. In: Cis/Transgene Optimization. SpringerBriefs in Systems Biology. Springer, Cham. doi:10.1007/978-3-319-90391-0_4
  9. Kadkhodaei S, Hashemi F, Rezaei M, et al. 2018. Outcomes Assessment. In: Cis/Transgene Optimization. SpringerBriefs in Systems Biology. Springer, Cham. doi: 10.1007/978-3-319-90391-0_3
  10. Kadkhodaei S, Hashemi F, Rezaei M, et al. 2018. Systematic Strategies. In: Cis/Transgene Optimization. SpringerBriefs in Systems Biology. Springer, Cham. doi: 10.1007/978-3-319-90391-0_2
  11. Ahmadi F, Abdullah SNA, Kadkhodaei S, Ijab S, Abdul Aziz M, Rahmane Z, Alwee SS. 2018. Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems. Plant Physiology and Biochemistry, 127, 320–335. doi: 10.1016/j.plaphy.2018.04.004
  12. Tan JS, Abbasiliasi S, Kadkhodaei S, Tam YJ, Tang T-K, Lee Y-Y, Ariff AB. 2018. Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli. BMC Microbiology 18. doi: 10.1186/s12866-017-1145-9
  13. Movahedi A, Zhang J, Sun W, Kadkhodaei S, Mohammadi K. 2018. Plant small RNAs: definition, classification and response against stresses. Biologia 1-10. doi: 10.2478/s11756-018-0034-5
  14. Abbasiliasi S, Tan JS, Ibrahim TAT, Ramanan RN, Kadkhodaei S, Mustafa S, Ariff AB. 2018. Kinetic modeling of bacteriocin-like inhibitory substance secretion by Pediococcus acidilactici Kp10 and its stability in food manufacturing conditions. Journal of Food Science and Technoogyl. doi: 10.1007/s13197-018-3037-x
  15. Yeng S, Abbasiliasi S, Keong C, Kadkhodaei S, Shamzi M, Hashim R, Shun J. 2018. Extraction of fresh banana waste juice as non-cellulosic and non-food renewable feedstock for direct lipase production. Renewable Energy 126:431–436. doi: 10.1016/j.renene.2018.03.050
  16. Kadkhodaei S, Memari HR, Abbasiliasi S, Rezaei MA, Movahedi A, Tan JS, Ariff AB. 2016. Multiple overlap extension PCR (MOE-PCR): An effective technical shortcut to high throughput synthetic biology. RSC Advances. Royal Society of Chemistry. DOI: 10.1039/C6RA13172G.
  17. Sofijan H, Joo Shun T, Abbasiliasi S, Mustafa S, Puspaningsih N, Kadkhodaei S, Ariff AB. 2016. Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system. Separation Science and Technology. DOI: 10.1080/01496395.2016.1268159
  18. Bande F, Arshad SS, Bejo MH, Kadkhodaei S, Omar AR. 2016. Prediction of B-cells and T-cells epitopes in the spike glycoprotein of M41 and CR88 avian infectious bronchitis virus strains for application in peptide vaccine. Advances in Bioinformatics, DOI:10.1155/2016/5484972.
  19. Bamaiyi PH, Izreen MS, Khatijah M, Eizzati BH, Bainum CR, Farhana MK, Norfaizul MN, Kadkhodaei S, Khan GK. 2016. Isolation and polymerase chain reaction identification of bacteria from the 2014–2015 flood of Kota Bharu, Kelantan, Malaysia. Asian Biomedicine, 10:6, 549–565. DOI: 10.5372/1905-7415.1006.525
  20. Abbasiliasi S, Tan JS, Kadkhodaei S, Nelofer R, Ibrahim TAT, Mustafa S, Ariff AB. 2016. Enhancement of BLIS production by Pediococcus acidilactici kp10 in optimized fermentation conditions using an artificial neural network. RSC Advances. 6 (8), 6342-6349.
  21. Kadkhodaei S, Barantalab F, Taheri S, Foroughi M, Hashemi FG, Shabanimofrad MR, et al. 2015. BioInfoBase: A Bioinformatics Resourceome. arXiv: Cornel University Library. 1607.02974v1.
  22. Kadkhodaei S, Abbasiliasi S, Tan JS, Fard Masoumi HR, Mohamed MS, Rahim R, Ariff AB. 2015. Enhancement of protein production by microalgae Dunaliella salina under mixotrophic condition using response surface methodology. RSC Advances. DOI: 10.1039/C5RA04546K.
  23. Movahedi A, Zhang J, Gao P, Yang Y, Wang L, Yin T, Kadkhodaei S, Ebrahimi M, Zhuge Q. 2014. Expression of the chickpea CarNAC3 gene enhances salinity and drought tolerance in transgenic poplars. Plant Cell, Tissue and Organ Culture. 1-14.
  24. Mohamed MS, Tan JS, Kadkhodaei S, Mohamad R, Mokhtar MN, Ariff AB. 2014. Kinetics and modeling of microalga Tetraselmis sp. FTC 209 growth with respect to its adaptation toward different trophic conditions. Biochemical Engineering Journal. 88:30–41.
  25. Tan JS, Abbasiliasi S, Lin YK, Mohamed MS, Kapri MR, Kadkhodaei S, Tam YJ, Rahman RNZRA, Ariff AB. 2014. Primary recovery of thermostable lipase 42 derived from recombinant Escherichia coli BL21 in aqueous two-phase flotation. Separation and Purification Technology. 133:328–334.
  26. Abbasiliasi S, Tan JS, Ibrahim TAT, Kadkhodaei S, Ng HS, Vakhshiteh F, Ajdari Z, Mustafa S, Ling TC, Rahim RA, Ariff AB. 2014. Primary recovery of a bacteriocin-like inhibitory substance derived from Pediococcus acidilactici Kp10 by an aqueous two-phase system. Food Chemistry. 151:93–100.
  27. Ghaderi-Zefrehei M, Memari H, Kadkhodaei S. 2010. Multilevel modeling in human microarray time course gene expression data. 1-5. doi: 10.1109/ICBME.2010.5705026.
  28. Ebrahimi M, Pezashki A, Nekouei MK, Kadkhodaei S. 2013. Investigation effect of kind and concentration of sodium alginate encapsulation in germination and growth of alfalfa artificial seed. Agronomy Journal (Pajouhesh & Sazandegi). 101: 48-59.
  29. Kadkhodaei S, Nekouei MK, Shahnazari M, Etminani H, Imani A, Ghaderi-Zefrehei M, Elahy M, Ariff AB. 2011. Molecular tagging of agronomic traits using simple sequence repeats: Informative markers for almond (Prunus dulcis) molecular breeding. Australian Journal of Crop Science.  5:1199–1209.
  30. Kadkhodaei S, Shahnazari M, Nekouei MK, Ghasemi M, Etminani H, Imani A, Ariff AB. 2011. A comparative study of morphological and molecular diversity analysis among cultivated almonds (Prunus dulcis). Australian Journal of Crop Science. 5:82–91.
  31. Kadkhodaei S, Elahy M, Nekouei MK, Imani A, Shahnazari M, Mardi M, Javanmard A, Ariff AB. 2010. A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis). Australian Journal of Crop Science. 4:730–736.
  32. Pezeshki A, Ebrahimi M, Nekouei MK, Kadkhodaei S. 2010. Effects of several plant growth regulators, carbon source and amino acids on somatic embryos obtained from embryogenic cell suspension culture of alfalfa (Medicago sativa L.). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 18(1):63-73.
  33. Ebrahimi M, Nekouei MK, Kadkhodaei S. 2009. Study of Some Factors Influencing Embryogenesis and Maturation of Soybean (Glycine max) Somatic Embryos. Journal of Water and Soil Science. 12(46): 167-176.
  34. Fathi A, Ghareyazi B, Haghnazari A, Ghaffari MR, Pirseyedi SM, Kadkhodaei S, Naghavi MR, Mardi M. 2008. Assessment of the genetic diversity of almond (Prunus dulcis) using microsatellite markers and morphological traits. Iranian Journal of Biotechnology. 6(2):98-106.
  35. Kadkhodaei S, Aghdaei SRT, Grigorian V, Moghadam M, Hashemi SMM. 2006. A study on genetic variation among some wild almond species using RAPD markers. Acta Hortic 93–98.
  36. Siahmard M, Daneshvar MH, Memari HR, Ardekani MR, Kadkhodaei S. 2012. Cloning of human gama INF in an expression vector for microalgae transformation. Modern Genetics Journal. 4:10.
  37. Kadkhodaei S, Aghdaei RT. 2002. Optimizing DNA extraction procedure in case of Amygdalus spp.: A simple and inexpensive method for nucleic acid isolation from plants with high polysaccharides and polyphenolic compounds. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 10(10):15-27.
  38. Kadkhodaei S, Tabaei Aghdaei SR, Gerigourian V, Moghadam Mohammad M. 2002. Genetic diversity analysis among wild almond populations of Esfahan province using RAPD markers. Iranian Journal of Horticultural Science and Technology. 3(4):25-36.

 

Conferences:

  1. National Conference on chocolate, coffee and natural sweeteners-Keynote paper, 2019, National / Iran- Stevia, the prospect of using natural sweeteners to promote community health
  2. 2nd International Conference on Research in Life-Sciences & Healthcare (ICRLSH), 2018, International / Budapest- Development of SSR Markers Associated with Biosynthesis Pathway of Steviol Glycosides in Stevia through De Novo Transcriptome Assembly
  3. 2nd International Biotechnology Congress of Iran, 2017, International / Iran- Transcriptome Profiling of Drought Stress in Rice (Oryza sativa) Using cDNA-AFLP
  4. International Conference on Agricultural and Food Engineering (Cafei2016), 2016, International / Malaysia- Molecular approach for enhancing phosphate uptake in oil palm
  5. Invited scientific talk, 2016, National / Iran- Cis/transgene optimization through bioinformatics approaches
  6. The NanoMITe Annual Symposium (NMAS 2016), 2016, National / Malaysia- Characterization of Defense Response Genes During Early Interaction of Oil Palm (Elaeis Guineensis Jacq.) with Ganoderma Boninense
  7. 3rd Plant Genomics Congress Asia, 2016, International / Malaysia- In silico analysis of oil palm phosphate-starvation-inducible PHT1 gene
  8. International Association for Plant Biotechnology, IAPB Congress, Melbourne, Australia, 2014, International / Australia- Downstream optimization of microalgae mixotrophic cultivation using response surface methodology for increasing protein production
  9. International Association for Plant Biotechnology, IAPB Congress, 2014, International / Australia- BioInfoBase: A bioinformatics resourceome
  10. International Association for Plant Biotechnology, IAPB Congress, Melbourne, Australia, 2014, International / Australia- cDNA-AFLP transcriptomic profiling of upland and lowland rice (Oryza sativa) under water deficit
  11. The 13th Iranian & The Second International Congress of Microbiology, 2012, International / Iran- Cloning of bialaphos resistant gene in expression vector for gene transformation to Dunaliella salina
  12. International Conference on Biomedical Engineering and Technology. IPCBEE vol.11 © IACSIT Press, Singapore, 2011, International / Malaysia- Construction of an expression vector for production of tissue plasminogen activator (t-PA) in a transgenic microalgae bioreactor
  13. The 1st Iranian Students Scientific Conference in Malaysia, 2011, International / Malaysia- Expression vector for production of recombinant proteins in a transgenic microalgae bioreactor
  14. 17th Iranian Conference of Biomedical Engineering, ICBME 2010, 2010, International / Iran- Multilevel modeling in human microarray time course gene expression data
  15. 2nd International Biotechnology & Biodiversity Conference (BIOJOHOR 2010), 2010, International / Malaysia- Construction of a specific expression vector for development of a transgenic Dunaliella salina bioreactor
  16. 3rd Iranian Conference on Bioinformatics, 2010, National / Iran- Potential of Microsoft Excel in developing bioinformatics softwares
  17. 5th International Symposium on Pistachios and Almonds, 2009, International / Turkey- A study on the presence of self-compatibility alleles in Iranian wild almonds (Amygdalus spp.) using specific allele amplification
  18. 5th International Symposium on Pistachios and Almonds, 2009, International / Turkey- Assessment of the genetic diversity of almond (Prunus dulcis) using microsatellite markers and morphological traits
  19. 5th International Symposium on Pistachios and Almonds, 2009, International / Turkey- Development of a molecular identification key for the most important Iranian almond cultivars through DNA fingerprinting
  20. 5th International Symposium on Pistachios and Almonds, 2009, International / Turkey- Development of a molecular identification database for Iranian cultivated almonds (Prunus dulcis)
  21. 5th International Symposium on Pistachios and Almonds, 2009, International / Turkey- Identification of candidate alleles as informative markers for some traits of almond (Prunus dulcis)
  22. 5th International Symposium on Pistachios and Almonds, 2009, International / Turkey- Karyotype analysis of three wild Iranian almond species (Amygdalus spp.)
  23. 5th International Symposium on Pistachios and Almonds, 2009, International / Turkey- Phenotypic diversity and morphological characterization of some important Iranian almonds
  24. 6th Iranian National Congress of Biotechnology, 2009, National / Iran- Identification, cloning and characterization of the phenylalanine amino mutase gene from Taxus baccata involved in Taxol production
  25. 18th EUCARPIA General Congress, 2008, International / Spain- ABRIISTAT 30: molecular data analysis software for genetic diversity studies
  26. 10th Iranian Genetic Congress, 2008, National / Iran- DNA fingerprinting of Iranian important fruit trees
  27. 10th Iranian Genetic Congress, 2008, National / Iran- Karyotype analysis of some Iranian wild almonds
  28. 10th Iranian Genetic Congress, 2008, National / Iran- Identification of informative markers for some fruit traits of almond
  29. 5th Iranian National Congress of Biotechnology, 2007, National / Iran- Introduction of Agricultural Biotechnology Multimedia Database
  30. 5th Iranian National Congress of Biotechnology, 2007, National / Iran- Genetic identification of Iranian almond cultivars using molecular markers
  31. 5th Iranian National Congress of Biotechnology, 2007, National / Iran- Effects of some factors on quality and quantity of somatic embryos obtained from alfalfa (Medicago sativa L.) suspension culture
  32. 5th Iranian National Congress of Biotechnology, 2007, National / Iran- Optimization of alfalfa (Medicago sativa L.) somatic embryo encapsulation stage in order to production of synthetic seed
  33. 5th Iranian National Congress of Horticulture, 2007, National / Iran- Study of heat requirement for flowering among the most important almond cultivars
  34. 5th Iranian National Congress of Horticulture, 2007, National / Iran- Genetic variation among some Iranian and foreign almond cultivars using SSR markers
  35. 14th National & 2nd International Conference of Biology, 2007, International / Iran- A study on some factors affecting somatic embryogenesis in soybean (Glycine max L.)
  36. 14th National & 2nd International Conference of Biology, 2007, International / Iran- Application of activated charcoal in DNA extraction procedures in order to remove polyphenolic compounds from plants tissues
  37. IV International Symposium on Pistachios and Almonds, 2006, International / Iran- A preliminary study on genetic diversity of some almond cultivars using RAPD markers
  38. IV International Symposium on Pistachios and Almonds, 2006, International / Iran- A simple and inexpensive method for nucleic acid isolation from plants with high polysaccharides and polyphenolic compounds
  39. 4th Iranian national congress of Biotechnology, 2005, National / Iran- Evaluation of genetic diversity of some almond cultivars using molecular and morphological data
  40. 1st National Congress of genetic resources, 2003, National / Iran- A study on almond genetic resources in Isfahan province
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